"In future posts in this series, I will cover other mechanisms of spike protein toxicity as well as other mechanisms of vaccine side effects that do not involve direct toxicity of the spike protein, and will brainstorm actionable steps vaccinees can take to protect against these effects or heal from them."
We'll certainly look forward to those thoughts. Do you have information on effects of spike protein among vaccinees lingering in lymph nodes for several months, the mechanism for their elimination, assuming that's what is needed, and how they end up there in the first place. Thanks, Howard Calvert
I've purchased many of your other pieces of work - this particular one was mind-blowing analysis (appreciated) and makes a lot of sense connecting dots around mucus membranes, loss of proteins that create junctions, mitochondrial and energy function that I've wondered about since the pandemic started. Also appreciated your muscle function example in point 2 in Additional notes... (tied into my nephew's rare neurodegenerative disease actually) - I can't wait for ongoing work you mention here and how to heal from or prevent and symptoms. This could explain a lot of what's happening to me over time since my vaccine that I've had no explanation for and you start to feel a little (crazy) and just stop talking about it to others as symptoms migrate to different places. Demonstrates "long-haul" isn't just for those that had covid (or maybe had it and went undetected) but also for some that got the vaccine. In addition, are now seeing young people getting blood clots, others in their 40's or 50's just dying suddenly of heart trouble - I'm not sure these things are random - there are too many coincidences showing up on the radar - it's becoming too frequent and within age groups that it didn't happen to before.
Fantastic work! I had been wondering about how the changes to the vax spike affected ACE2 binding. Great finds. Btw I'm certain the concentrations of S1 as measured by that Ogata study are underestimates of total S1 that can cause trouble since the assay they used wouldn't have picked up any spike bound to antibodies or on the surface of exosomes, etc. More on that here: https://joomi.substack.com/p/what-leads-to-more-free-spike-protein
I am a PhD in science myself so i am all for analysis and information. But i cant shake the sense here that youre spreading fear - for free - and then charging money for a promised solution to the fear and problem you create!
Why not publish so we can see some expert rebuttals to clains like 4 month duration of spike protein persistence?.
I know journals are odd about what covid papers they accept. Ive got a covid paper i wrote stuck on a preprint server because the oddest reviews i have ever seen prevented its publication. It was treatment oriented but backed of course by science. Still tho, even preprint server would allow more professionals to comment on the specifics then here
I am not vaxed because i have MCAS, cfs, a rheumatologicalmAI disease , and a recurrent hepes virus that i think has already poked holes in my vascular system. Still tho, despite my own hesitations reading your post has made it harder for me, more fear mongering and inalready have plenty and made , objective facts harder to evaluate in my own daily reassessment of covid infection risk vs covid vax. Eg to evaluate cost benefit risk of vax, you need to know your risk of contracting covid. So noninfection better than vaccine but is vaccine better then covid infection? These issues need discussion.
I hope to see your work in more peer reviewed outlets. For now im skeptical you try to make money by making people afraid. Abd we will see if youvallow this to be posted
Is it likely in your opinion Chris that this damage could be going on for a year after initial mRNA series? My poor Mom has never regained her energy after completing her initial COVID vaccine series. I would especially love to hear your early thoughts on how to heal from this. My 82 year old mom is losing the desire to live in such a weak state. We are desperate for a solutions.
I think you messed up the math by a factor of 1000.
Your text said '...calculated on the basis that commercial S1 protein is 75 grams per mole.' and the link you provided there states Molecular Weight of S1 is 75 kDa, which is 75 kg / mol (kilogram per mole).
That would mean all of the concentrations you calculated are 1000 times smaller (not nM, but pM).
I am a PhD in science myself so i am all for analysis and information. But i cant shake the sense here that youre spreading fear - for free - and then charging money for a promised solution to the fear and problem you create!
Why not publish so we can see some expert rebuttals to clains like 4 month duration of spike protein persistence?.
I know journals are odd about what covid papers they accept. Ive got a covid paper i wrote stuck on a preprint server because the oddest reviews i have ever seen prevented its publication. It was treatment oriented but backed of course by science. Still tho, even preprint server would allow more professionals to comment on the specifics then here
I am not vaxed because i have MCAS, cfs, a rheumatologicalmAI disease , and a recurrent hepes virus that i think has already poked holes in my vascular system. Still tho, despite my own hesitations reading your post has made it harder for me, more fear mongering and inalready have plenty and made , objective facts harder to evaluate in my own daily reassessment of covid infection risk vs covid vax. Eg to evaluate cost benefit risk of vax, you need to know your risk of contracting covid. So noninfection better than vaccine but is vaccine better then covid infection? These issues need discussion.
I hope to see your work in more peer reviewed outlets. For now im skeptical you try to make money by making people afraid. Abd we will see if youvallow this to be posted
"Hence, the spike protein is consistently found in the lymph nodes of the arm pits at least 60 days after vaccination, but traces are rarely found at all after natural infection."
As far as I can tell, no one has looked. https://www.science.org/doi/10.1126/sciimmunol.abl9105 is a very scant/uneven glimpse into the post-infection lymph node situation and did not stain for spike itself, just reactive GC B Cells / TFH. But still found " GCs being active for months after infection" (Fig 5) which would imply said active germinal centers still have the spike protein in FDCs. Röltgen, et al. infected sample set was for critical infections, and GCs seem to be disorganized / disordered, so they aren't a good comparison.
There's a lot of overlap with a pore-forming toxicity model and Wentzel's kynurenine pathway model, including overlap with anaerobic metabolism - https://youtu.be/ZFPleh6z7io . (But I'm still not clear on what he proposes as the mechanism besides "inflammation.") On the other hand, nothing about the S1 really looks like it could effectively polymerize into a cylinder.
"This is shown by making synthetic lipid bilayers permeable to charged ions."
This suggests that spike proteins would facilitate zinc entry into cells without the need for ionophores. Or does this only happen when a spike protein impacts the cell membrane without being part of a virus?
Eight individuals is a very small sample. I question whether all vaccinated individuals show the same exosomal half life as these eight. Usually there is a range. This has to be preliminary, although it is a signal that caution should be exercised. Of course, the signal will be ignored by the FDA.
"In future posts in this series, I will cover other mechanisms of spike protein toxicity as well as other mechanisms of vaccine side effects that do not involve direct toxicity of the spike protein, and will brainstorm actionable steps vaccinees can take to protect against these effects or heal from them."
We'll certainly look forward to those thoughts. Do you have information on effects of spike protein among vaccinees lingering in lymph nodes for several months, the mechanism for their elimination, assuming that's what is needed, and how they end up there in the first place. Thanks, Howard Calvert
I've purchased many of your other pieces of work - this particular one was mind-blowing analysis (appreciated) and makes a lot of sense connecting dots around mucus membranes, loss of proteins that create junctions, mitochondrial and energy function that I've wondered about since the pandemic started. Also appreciated your muscle function example in point 2 in Additional notes... (tied into my nephew's rare neurodegenerative disease actually) - I can't wait for ongoing work you mention here and how to heal from or prevent and symptoms. This could explain a lot of what's happening to me over time since my vaccine that I've had no explanation for and you start to feel a little (crazy) and just stop talking about it to others as symptoms migrate to different places. Demonstrates "long-haul" isn't just for those that had covid (or maybe had it and went undetected) but also for some that got the vaccine. In addition, are now seeing young people getting blood clots, others in their 40's or 50's just dying suddenly of heart trouble - I'm not sure these things are random - there are too many coincidences showing up on the radar - it's becoming too frequent and within age groups that it didn't happen to before.
Great information for the vaxxed and unvaxxed. Please see this link: https://pubmed.ncbi.nlm.nih.gov/34461999/
Fantastic work! I had been wondering about how the changes to the vax spike affected ACE2 binding. Great finds. Btw I'm certain the concentrations of S1 as measured by that Ogata study are underestimates of total S1 that can cause trouble since the assay they used wouldn't have picked up any spike bound to antibodies or on the surface of exosomes, etc. More on that here: https://joomi.substack.com/p/what-leads-to-more-free-spike-protein
Very interesting. Thank you
I am a PhD in science myself so i am all for analysis and information. But i cant shake the sense here that youre spreading fear - for free - and then charging money for a promised solution to the fear and problem you create!
Why not publish so we can see some expert rebuttals to clains like 4 month duration of spike protein persistence?.
I know journals are odd about what covid papers they accept. Ive got a covid paper i wrote stuck on a preprint server because the oddest reviews i have ever seen prevented its publication. It was treatment oriented but backed of course by science. Still tho, even preprint server would allow more professionals to comment on the specifics then here
I am not vaxed because i have MCAS, cfs, a rheumatologicalmAI disease , and a recurrent hepes virus that i think has already poked holes in my vascular system. Still tho, despite my own hesitations reading your post has made it harder for me, more fear mongering and inalready have plenty and made , objective facts harder to evaluate in my own daily reassessment of covid infection risk vs covid vax. Eg to evaluate cost benefit risk of vax, you need to know your risk of contracting covid. So noninfection better than vaccine but is vaccine better then covid infection? These issues need discussion.
I hope to see your work in more peer reviewed outlets. For now im skeptical you try to make money by making people afraid. Abd we will see if youvallow this to be posted
Is it likely in your opinion Chris that this damage could be going on for a year after initial mRNA series? My poor Mom has never regained her energy after completing her initial COVID vaccine series. I would especially love to hear your early thoughts on how to heal from this. My 82 year old mom is losing the desire to live in such a weak state. We are desperate for a solutions.
I think you messed up the math by a factor of 1000.
Your text said '...calculated on the basis that commercial S1 protein is 75 grams per mole.' and the link you provided there states Molecular Weight of S1 is 75 kDa, which is 75 kg / mol (kilogram per mole).
That would mean all of the concentrations you calculated are 1000 times smaller (not nM, but pM).
Igor Sviben PhD Chemistry, University of Zagreb
I am a PhD in science myself so i am all for analysis and information. But i cant shake the sense here that youre spreading fear - for free - and then charging money for a promised solution to the fear and problem you create!
Why not publish so we can see some expert rebuttals to clains like 4 month duration of spike protein persistence?.
I know journals are odd about what covid papers they accept. Ive got a covid paper i wrote stuck on a preprint server because the oddest reviews i have ever seen prevented its publication. It was treatment oriented but backed of course by science. Still tho, even preprint server would allow more professionals to comment on the specifics then here
I am not vaxed because i have MCAS, cfs, a rheumatologicalmAI disease , and a recurrent hepes virus that i think has already poked holes in my vascular system. Still tho, despite my own hesitations reading your post has made it harder for me, more fear mongering and inalready have plenty and made , objective facts harder to evaluate in my own daily reassessment of covid infection risk vs covid vax. Eg to evaluate cost benefit risk of vax, you need to know your risk of contracting covid. So noninfection better than vaccine but is vaccine better then covid infection? These issues need discussion.
I hope to see your work in more peer reviewed outlets. For now im skeptical you try to make money by making people afraid. Abd we will see if youvallow this to be posted
"Hence, the spike protein is consistently found in the lymph nodes of the arm pits at least 60 days after vaccination, but traces are rarely found at all after natural infection."
As far as I can tell, no one has looked. https://www.science.org/doi/10.1126/sciimmunol.abl9105 is a very scant/uneven glimpse into the post-infection lymph node situation and did not stain for spike itself, just reactive GC B Cells / TFH. But still found " GCs being active for months after infection" (Fig 5) which would imply said active germinal centers still have the spike protein in FDCs. Röltgen, et al. infected sample set was for critical infections, and GCs seem to be disorganized / disordered, so they aren't a good comparison.
There's a lot of overlap with a pore-forming toxicity model and Wentzel's kynurenine pathway model, including overlap with anaerobic metabolism - https://youtu.be/ZFPleh6z7io . (But I'm still not clear on what he proposes as the mechanism besides "inflammation.") On the other hand, nothing about the S1 really looks like it could effectively polymerize into a cylinder.
"S1 and S2 probably both act as direct, pore-forming toxins at concentrations as low as 0.1 nM."
The units of concentration include volume in the denominator. Can't tell if this is nM/Liter or nM/ml.
"the inflammatory response attacking the proteins at the cell junctions."
Mast cells?
"This is shown by making synthetic lipid bilayers permeable to charged ions."
This suggests that spike proteins would facilitate zinc entry into cells without the need for ionophores. Or does this only happen when a spike protein impacts the cell membrane without being part of a virus?
Eight individuals is a very small sample. I question whether all vaccinated individuals show the same exosomal half life as these eight. Usually there is a range. This has to be preliminary, although it is a signal that caution should be exercised. Of course, the signal will be ignored by the FDA.